Breakthrough in Artificial Cell Technology: Model Nuclei Mass-Produced via Microfluidics.

A team of researchers led by Professor Hiroaki Suzuki from the Faculty of Science and Engineering at Chuo University has successfully developed a method to mass-produce artificial cells containing model nuclei, using microfluidic devices with high consistency and reproducibility. The findings, published in JACS Au, mark a significant advancement in artificial cell engineering.

The research contributes to the global effort to construct artificial cells from the bottom up by combining biomolecular components such as lipids, proteins, and DNA. Recently, particular attention has been directed toward replicating the complex architecture of eukaryotic cells—especially the nucleus, the largest and most vital organelle that houses condensed genomic DNA.

Building on their earlier work in creating uniform artificial cells with microfluidic systems, the team encapsulated materials necessary for DNA condensation—specifically DNA nanostars and salt—into uniformly sized lipid bilayer vesicles. Through osmotic manipulation, they induced volume changes in these vesicles, effectively concentrating the ingredients and forming consistent model nuclei.

Traditionally, thermal annealing has been used to create DNA condensates, but this process risks damaging other sensitive components within artificial cells. The researchers overcame this challenge by using a gentler method of concentration control, preserving the functional integrity of embedded enzymes and reaction systems. Impressively, they achieved successful synthesis of green fluorescent protein (GFP) from genes embedded within the artificial nuclei.

These newly developed artificial cells are expected to possess advanced functions such as environmental responsiveness and molecular recognition. The study represents a promising step toward the future creation of artificial cells capable of mimicking—and potentially replacing—natural living cells.

Source:https://phys.org/news/2025-06-artificial-cells-nuclei-mass-microfluidic.html

This is non-financial/medical advice and made using AI so could be wrong.